The Journal of Biological Chemistry, Volumes 17-18

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American Society for Biochemistry and Molecular Biology, 1914
Vols. 3-140 include the society's Proceedings, 1907-41

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Page 325 - WAKEMAN. (Prom the Laboratory of the Connecticut Agricultural Experiment Station and the Sheffield Laboratory of Physiological Chemistry in Yale University, New Haven...
Page 154 - ... amorphous powder. It was free from inorganic phosphate, and bases other than barium could not be detected. But it still contained oxalates from which it was freed in the manner described in a former paper.7 The substance was finally precipitated from about 1 per ct. hydrochloric acid with alcohol, filtered and washed free of chlorides with dilute alcohol and then in alcohol and ether and dried in vacuum over sulphuric acid. The dry preparation weighed about 66 grams. This crude acid salt was...
Page 181 - Of this acid, 12.8 grams (correspending to 10 grams of the dry substance) were dissolved in about 500 cubic centimeters of water and barium hydroxide (Kahlbaum, alkali free) added to slight alkaline reaction. The precipitate was filtered and washed several times in water. The barium precipitate was reserved for special examination. The filtrate was examined for inosite as follows: The excess of barium hydroxide was precipitated with carbon dioxide, filtered and evaporated on the water bath nearly...
Page 378 - The method to be described depends on a new principle — the determination of the fat by precipitation in a water solution and comparison of the cloudy suspension so obtained with that of a similarly prepared standard fat solution by the use of the nephelometer. The determination may be completed in about threequarters of an hour and may be carried out with from 0.5 cc to 5 cc of blood. Ordinarily about 2 cc are used. It has been found to be accurate to within 5 per cent, of the total fat. The procedure...
Page 183 - The resulting precipitate was filtered, washed free of chlorides with dilute alcohol and then hi absolute alcohol and ether and dried in vacuum over sulphuric acid. The substance was then a pure white, amorphous powder which showed a strong acid reaction on moist litmus paper. It was analyzed after drying at 105° in vacuum over phosphorus pentoxide.
Page 295 - BY FRANK P. UNDERBILL. (From the Sheffield Laboratory of Physiological Chemistry, Yale University, New Haven, Conn...
Page xxx - In advanced osteitis deformans the first step in the new formation of bone or osteoid tissue may be the production of a highly sulphurized organic matrix, which is transformed gradually by a calcification process which is accompanied by the deposition of Ca, Mg, and P in this matrix. In the course of this calcification procedure we may suppose that a certain quota of the sulphur of the matrix is replaced by the other elements mentioned, a process which must entail the retention of Ca, Mg, and P and...
Page 146 - Ba = 47.07 per ct. The substance was again dissolved in 1 per ct. hydrochloric acid and the solution neutralized to litmus with barium hydroxide. It was filtered, washed free of chlorides with water and then in alcohol and ether and dried in vacuum over sulphuric acid. It was analyzed after drying as before and the following results obtained : 0.3168 gm. subst. gave 0.0481 gm. H20 and 0.0972 gm.
Page 520 - Of 34 second-year medical students examined before and after an examination, 1 had sugar both before and after the examination. Of the remaining 33, 6, or 18 per cent., had small but unmistakable traces of sugar in the urine passed immediately after the examination. A similar study was made on second-year women students at Simmons College. Since these students were younger and presumably much more excitable than our medical students it was thought that even more striking results might be obtained....
Page 167 - ... of removing adhering inorganic phosphate was precipitated twice from 0.5 per ct. hydrochloric acid, in the presence of a small quantity of barium chloride, with alcohol and then twice from the same strength hydrochloric acid with alcohol. After finally filtering and thoroughly washing in 50 per ct alcohol, alcohol and ether it was dried in vacuum over sulphuric acid. The product so obtained was a white amorphous powder. In appearance it was very similar to the tribarium phytate and the barium...

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