The Journal of Biological Chemistry, Volume 237, Pages 1381-2045

1-phosphate a-D-glucose absorption acetate acetyl-CoA Acta added addition aliquot amino acid ammonium sulfate amount approximately assay B-sitosterol Biochem Biol Biophys cells centrifugation Chem chromatography coenzyme column compounds concentration containing crystalline crystallization Curve cytochrome D-glucose dehydrogenase denaturation described determined DPNH EDTA eluate eluted enzymatic enzyme enzyme preparation ester estradiol estriol ethanol ether ethyl experimental experiments extract fatty acid fraction galactose gland glucose glutathione groups hexose hydrolysis hydroxyproline incorporation incubation indicated inhibition iodoacetic acid isolated kinase labeled lactose liver malonyl-CoA method microsomes minutes mole N-acetyl-D-mannosamine nucleotide obtained oxidation paper paper chromatography peptide phate phosphate buffer polylysine precipitate present procedure protein purified pyruvate radioactivity reaction mixture reagent reduced reductase residue RNase sample shown sodium soluble solution solvent specific activity steroids sterol studies substrate supernatant synthesis Table tetrahydrofuran tion TPNH triterpene tubes tyrosine urea values volume